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Series GSE47495 Query DataSets for GSE47495
Status Public on Nov 15, 2013
Title Transcriptional profiling of left ventricle and peripheral blood cells in rats with post-myocardial infarction
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Myocardial infarction (MI) often results in left ventricular (LV) remodeling followed by heart failure (HF). It is of great clinical importance to understand the molecular mechanisms that trigger transition from compensated LV injury to HF and to identify relevant diagnostic biomarkers. In this study, we performed transcriptional profiling of LVs in rats with a wide range of experimentally induced infarct sizes and of peripheral blood mononuclear cells (PBMCs) in animals that developed HF.
We used microarrays to investigate gene expression in the left ventricle (LV) accompanying myocardial infarction and concomitant heart failure (HF) in a well validated model of post-infarcted heart failure and to evaluate their reflection in peripheral blood mononuclear cells (PBMCs)
 
Overall design Myocardial infarction (MI) was induced in male Wistar rats by ligation of the proximal left coronary artery. The sham-operated group (control group) was subjected to the same protocol, except that the suture was not tied around the proximal left coronary artery. Sham-operated rats (n=6) and rats with small (n=6), moderate (n=6), and large (n=5) MI size were included into the experiment two months after the operation. Then, left ventricules and blood samples were obtained for RNA extraction and hybridization on Affymetrix microarrays. Microarrays were used to compare the LV and PBMCs transcriptomes of control and experimental animals. The development of heart failure was estimated by echocardiography and catheterization.
 
Contributor(s) Tulacz D, Burzynska B, Gora M, Mackiewicz U, Maczewski M
Citation(s) 24206753
Submission date May 29, 2013
Last update date Nov 15, 2013
Contact name Dorota Anna Tulacz
E-mail(s) dtulacz@ibb.waw.pl
Organization name Institute of Biochemistry and Biophysics of the Polish Academy of Sciences
Department Department of Genetics
Street address Pawinskiego 5a
City Warszawa
ZIP/Postal code 02-106
Country Poland
 
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (34)
GSM1151154 hearts from sham-operated rat, biological rep1
GSM1151155 hearts from sham-operated rat, biological rep2
GSM1151156 hearts from sham-operated rat, biological rep3
Relations
BioProject PRJNA205867

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47495_RAW.tar 140.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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