|
| Status |
Public on May 23, 2013 |
| Title |
Ribosome Profiling over a Zebrafish Developmental Timecourse |
| Organism |
Danio rerio |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
To experimentally-validate the non-coding status of annotated lncRNAs, we performed ribosome profiling over a developmental timecourse that matched our previously-published (Pauli et al. 2012) developmental transcriptome. We find that many previously-annotated lncRNAs appear to be translated, but in a pattern more akin to 5' leaders of coding genes.
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| |
|
| Overall design |
Ribosome profiling over 8 stages in early zebrafish development: 2-4 cell, 256 cell, 1K cell, Dome, Shield, Bud, 28hpf and 5dpf
|
| |
|
| Contributor(s) |
Chew G, Pauli A, Valen E, Schier A |
| Citation(s) |
23698349 |
| Submission date |
Apr 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Guo-Liang Chew |
| E-mail(s) |
chew@fas.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Dept of Molecular and Cellular Biology
|
| Lab |
Schier
|
| Street address |
16 Divinity Ave BL 1019
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA200706 |
| SRA |
SRP021915 |
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