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Series GSE46283 Query DataSets for GSE46283
Status Public on Apr 24, 2013
Title Expression data of multiple sclerosis patients receiving Interferon-beta therapy [TaqMan(r) Array Human MicroRNA A Cards v2.0]
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary The purpose of this study was to investigate the expression dynamics of mRNAs and microRNAs in response to subcutaneous IFN-beta-1b treatment (Betaferon, 250 µg every other day) in patients with clinically isolated syndrome (CIS) suggestive of multiple sclerosis (MS) or relapsing-remitting type of the disease (RRMS).
By using TaqMan Array MicroRNA Cards, we obtained microRNA expression profiles of peripheral blood mononuclear cells from 6 patients within the first month of IFN-beta treatment.
 
Overall design EDTA blood samples were taken from all patients immediately before first, second and third IFN-beta injection as well as after 1 month.
Total RNA of Ficoll-isolated peripheral blood mononuclear cells from each sample was extracted. Real-time PCR measurements were performed in 384-well plates with TaqMan assay reagents to quantify the microRNA expression levels.
This GEO entry provides the data of the TaqMan Array Human MicroRNA A Cards v2.0.
 
Contributor(s) Hecker M, Koczan D, Zettl UK
Citation(s) 23921681
Submission date Apr 22, 2013
Last update date Aug 08, 2013
Contact name Michael Hecker
E-mail(s) michael.hecker@rocketmail.com
Organization name University of Rostock
Department Department of Neurology
Lab Division of Neuroimmunology
Street address Gehlsheimer Str. 20
City Rostock
ZIP/Postal code 18147
Country Germany
 
Platforms (1)
GPL13328 TaqMan(r) Array Human MicroRNA A Cards v2.0
Samples (24)
GSM1128112 Patient 1, before first IFN-beta injection [TaqMan(r) Array Human MicroRNA A Cards v2.0]
GSM1128113 Patient 1, before second IFN-beta injection [TaqMan(r) Array Human MicroRNA A Cards v2.0]
GSM1128114 Patient 1, before third IFN-beta injection [TaqMan(r) Array Human MicroRNA A Cards v2.0]
This SubSeries is part of SuperSeries:
GSE46293 Expression data of multiple sclerosis patients receiving Interferon-beta therapy
Relations
BioProject PRJNA198639

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46283.xls.gz 205.5 Kb (ftp)(http) XLS
Processed data included within Sample table
Processed data are available on Series record
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