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Series GSE45825 Query DataSets for GSE45825
Status Public on Apr 06, 2013
Title Transcriptional profiling of abdominal adipose tissue in juvenile broiler chickens divergently selected for high or low body weight
Organism Gallus gallus
Experiment type Expression profiling by array
Summary Chickens divergently selected for either high growth (HG genotype) or low growth (LG genotype) by F.H. Ricard at SRA-INRA, Nouzilly France were used for time-course transcriptional profiling of abdominal fat during juvenile development (1 to 11 weeks of age) to identify differentially expressed genes. The HG and LG chickens are different in various phenotypic and metabolic measurements, including growth rate, abdominal fat, plasma glycemia, insulinemia, T4, T3, triglyceride and NEFA. Thus, the HG and LG chickens are valuable as a model for biomedical and agricultural traits. The Del-Mar 14K Chicken Integrated Systems microarrays were used for a transcriptional scan in abdominal adipose during juvenile development using a balanced block hybridization design. Log2-transformed fluorescence intensities were analyzed with the linear mixed model (MIXED) procedure in SAS (SAS Institute Inc., Cary, NC, USA). A total of 3,957 differentially expressed functional genes were identified (FDR<0.05) as having a significant effect of age (2,918), genotype (312), or an age by genotype interaction (727). The differentially expressed genes include adipokines, metabolic enzymes, acute phase proteins, growth factors, coagulation factors, immune factors and transcription factors involved in various pathways. Keywords: abdominal fat, divergently selected chickens, growth, transcriptional profiling, differentially expressed genes, lipogenesis
 
Overall design A balanced block design was used for microarray hybridizations, where half of the birds of each genotype and age were labeled with Alexa Flour 647 (red) and the other half with Alexa Flour 555 (green). Four biological replicates were used for each genotype (HG or LG) at six different ages (1, 3, 5, 7, 9 and 11 wk).
 
Contributor(s) Resnyk CW, Carre W, Wang X, Simon J, Aggrey SE, Porter TE, Cogburn LA
Citation(s) 28814270
Submission date Apr 05, 2013
Last update date Aug 02, 2019
Contact name Larry Albert Cogburn
E-mail(s) cogburn@udel.edu
Phone 302-831-1335
Organization name University of Delaware
Department Animal and Food Sciences
Lab Avian Functional Genomics
Street address 531 South College Ave.
City Newark
State/province DE
ZIP/Postal code 19717
Country USA
 
Platforms (1)
GPL1731 DEL-MAR 14K Integrated Systems
Samples (24)
GSM1116050 Chicken Abdominal fat, high (HG) versus low (LG) growth lines, 1 week of age, replicate 1
GSM1116051 Chicken Abdominal fat, high (HG) versus low (LG) growth lines, 1 week of age, replicate 2
GSM1116052 Chicken Abdominal fat, high (HG) versus low (LG) growth lines, 1 week of age, replicate 3
Relations
BioProject PRJNA196583

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45825_RAW.tar 44.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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