|
| Status |
Public on May 20, 2014 |
| Title |
Expression data from BRAFV600E A375 melanoma cells treated with vehicle or vemurafenib |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Vemurafenib is a BRAF inhibitor with specificity for the most common BRAF mutant encountered in melanomas (BRAFV600E). Vemurafenib suppresses the proliferation of BRAF mutant human melanoma cells by suppressing downstream activation of the MEK/ERK mitogen activated protein kinases.
We used microarrays to examine the transcriptional response of a vemurafenib-sensitive BRAFV600E human melanoma cell line (A375) to vemurafenib in order to further delineate the mechanisms by which BRAFV600E drives cell proliferation and energy metabolism in human melanoma.
|
| |
|
| Overall design |
BRAFV600E A375 human melanoma cells were treated with vehicle (0.1% DMSO) or 10 μM vemurafenib for 24 h after which total RNA was extracted. Cells were prepared and RNA was extracted in 3 separate batches (three different cell stocks on three separate days) providing three independent replicates (n=3). Paired replicates (prepared from the same stock of cells on the same day) are denoted by A, B and C.
|
| |
|
| Contributor(s) |
Parmenter TJ, McArthur GA |
| Citation(s) |
24469106 |
| Submission date |
Dec 12, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Tiffany Joy Parmenter |
| E-mail(s) |
tiffanyparmenter@gmail.com
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
1 St Andrews Pl
|
| City |
East Melbourne |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA183688 |
Less...
More...