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| Status |
Public on Oct 30, 2012 |
| Title |
Hog1 bypasses stress-mediated down-regulation of transcription by PolII redistribution and chromatin remodeling |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Cells are subjected to dramatic changes on gene expression upon environmental changes. Stress causes a general down-regulation of gene expression together with the induction of a set of stress-responsive genes. Genome wide localisation studies showed major changes on Pol II localisation towards stress-responsive genes in contrast to housekeeping genes. Pol II relocalisation requires of the Hog1 SAPK, which also associates at stress-responsive loci. Chromatin structure was not significantly altered upon stress except for those genes that displayed Hog1 association. Together, Hog1 serves to bypass the general down-regulation of gene expression by targeting RNA Pol II machinery and inducing chromatin remodeling at stress-responsive loci.
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| |
|
| Overall design |
Hog1 and Pol II ChIP-Seq and Mnase-Seq experiments in both strains WT and Hog1 mutant, for 2 conditions: Exposed and not exposed to osmostress
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| |
|
| Contributor(s) |
Mariona N, Francesc P |
| Citation(s) |
23158682 |
| Submission date |
Oct 11, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Francesc Posas |
| Organization name |
UPF
|
| Street address |
Dr. Aiguader
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL13272 |
Illumina Genome Analyzer IIx (Saccharomyces cerevisiae) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA177335 |
| SRA |
SRP016070 |
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