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| Status |
Public on Sep 18, 2012 |
| Title |
Conversion of human fibroblasts into vascular cells (methylation) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
We report a novel technique to reprogram human fibroblasts into endothelial and smooth muscle cells using partial iPSC reprogramming and chemically defined media. Using appropriate media conditions for differentiation of human pluripotent cells to CD34+ vascular progenitor cells, we show that temporary expression of pluripotent transcription factors and treatment with chemically-defined media, will induce differentiation of human fibroblasts to CD34+ vascular progenitor cells. Sorted CD34+ cells can then be directed to differentiate into vascular endothelial cells expressing a variety of smooth muscle markers.
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| Overall design |
We have assessed the global DNA methylation (Illumina Infinium HD 450K DNA methylationBeadChips) and transcriptional (Illumina HT12v4 Gene Expression Bead Array) profiles of transdifferentiated endothelial cells and smooth muscle, human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) differentiated CD34+ angioblasts, hESCs, hiPSC, primary smooth muscle and primary human umbilical vein endothelial cells using microarrays.
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| Contributor(s) |
Kurian L, Sancho-Martinez I, Nivet E, Moon K, Pendaries C, Volle-Challier C, Bono F, Herbert J, Xia Y, Li M, Pulecio J, Monserrat N, Ruiz S, Aguirre A, Dubova I, Boscolo FS, Thiagarajan R, Loring JF, Laurent LC, Belmonte JC |
| Citation(s) |
23202434 |
| Submission date |
Sep 14, 2012 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Rathi D Thiagarajan |
| E-mail(s) |
rthiagarajan@ucsd.edu
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| Organization name |
UCSD
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| Department |
Reproductive Medicine
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| Lab |
Louise Laurent
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| Street address |
2880 Torrey Pines Scenic Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (25)
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| GSM1004628 |
trans.diff_4F.MIM_HFF637.1_p5_endothelial_630.1764.1.2 |
| GSM1004629 |
trans.diff_4F.mIR_HFF637.1_p4_endothelial_630.1765.1.2 |
| GSM1004630 |
trans.diff_4F.mIR_HFF637.1_p4_Smooth.Muscle_630.1766.1.2 |
| GSM1004631 |
trans.diff_4F_HFF637.1_p5_Smooth.Muscle_630.1767.1.2 |
| GSM1004632 |
hiPS_K633IPS1_p44_633.1773.1.2 |
| GSM1004633 |
hES_Hues9_p49_628.1763.1.2 |
| GSM1004634 |
hES.diff_Hues9_p4_Smooth.Muscle_628.1762.1.2 |
| GSM1004635 |
hiPS.diff_K633IPS1_pUNK_CD34pos.Angioblasts_633.1770.1.2 |
| GSM1004636 |
hES.diff_Hues9_pUNK_CD34pos.Angioblasts_628.1760.1.2 |
| GSM1004637 |
hiPS.diff_K633IPS1_p4_endothelial_633.1771.1.2 |
| GSM1004638 |
hES.diff_Hues9_p13_endothelial_628.1761.1.2 |
| GSM1004639 |
hiPS.diff_K633IPS1_p4_Smooth.Muscle_633.1772.1.2 |
| GSM1004640 |
Primary_SMOOTHMUSCLE_HBVSMC266_pUNK_203.556.1.1 |
| GSM1004641 |
Primary_SMOOTHMUSCLE_HESMC267_pUNK_207.560.1.1 |
| GSM1004642 |
Primary_SMOOTHMUSCLE_HSkMC268_pUNK_208.561.1.1 |
| GSM1004643 |
Primary_HUVEC_HUVECBF2_p4_168.249.2.1 |
| GSM1004644 |
Primary_HUVEC_HUVECWF8_p4_173.254.2.1 |
| GSM1004645 |
Primary_HUVEC_HUVEC632_p17_632.1769.1.2 |
| GSM1004646 |
hES_WA09_p41_87.304.7.2 |
| GSM1004647 |
hES_WA09_p43_87.521.4.1 |
| GSM1004648 |
hiPS.EARLY_HDF51IPS1_p6_153.437.1.1 |
| GSM1004649 |
hiPS.EARLY_HDF51IPS10_p6_161.445.2.1 |
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| This SubSeries is part of SuperSeries: |
| GSE40927 |
Conversion of human fibroblasts into vascular cells |
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| Relations |
| BioProject |
PRJNA175382 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40909_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE40909_methylation_unprocessed.txt.gz |
62.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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