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| Status |
Public on Jun 01, 2013 |
| Title |
Expression analysis of control and low protein fed mouse offspring subsequently treated with multiple low doses of STZ |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Low protein (LP) during gestation leads to low birth weight and poor fetal growth, with altered islet development and glucose intolerance in adulthood. Additionally, LP offspring fail to regenerate their β-cells following depletion with streptozotocin (STZ), in contrast to control-fed offspring that are capable of β-cell regeneration.
Our objective was to identify genes and signalling pathways that may be critically altered in LP offspring rendering them susceptible to develop long term glucose intolerance and decreased β-cell plasticity.
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| Overall design |
Pregnant Balb/C mice were place on a control (20% protein) or isocaloric low protein (8%) diet for the period of gestation, and then all dams were switch to the control diet at birth. Female offspring were injected with streptozotocin (35 mg/kg body weight; sodium citrate buffer 0.1 mol/L, pH 4.5) or vehicle (sham). Total RNA was extracted from pancreas of 30 day old female offspring (n=3) and hybridized to Affymetrix GeneChips.
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| Contributor(s) |
Cox AR, Carter D, Arany EJ, Hill DJ |
| Citation(s) |
23986224 |
| Submission date |
May 24, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Aaron R Cox |
| E-mail(s) |
arcox2@gmail.com
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| Organization name |
Baylor College of Medicine
|
| Department |
Pediatrics - Endocrinology and Diabetes
|
| Street address |
1102 Bates Ave.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77025 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA167451 |
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