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Series GSE37976 Query DataSets for GSE37976
Status Public on May 19, 2012
Title Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda)
Organism Lepeophtheirus salmonis
Experiment type Expression profiling by array
Summary To understand the sensitivity of larval lice to changing environmental parameters we applied a 38K oligo microarray and characterized transcriptome responses after 24 hour exposures to reduced salinity (30-10 parts per thousand (ppt)) or varied temperature (16-4ºC). To further characterize the effects of salinity, we profiled expression changes over a range of seawater with single increment differences (30-25 ppt).
 
Overall design Three separate projects. Low resolution salinity (aka wide range or LR_SAL) contains 2 duplicate experiments, each with pools of lice (~500 copepodid lice per beaker; n=3 beakers per condition), incubated for 24 hr at 10ºC diluted to 30, 25, 20, or 10 parts per thousand (ppt). Temperature study conducted as above, but with a constant salinity of 30 ppt and changed temperature of 4, 10, or 16ºC. High resolution salinity conducted as above, but with no experiment replication, but with biological repication of n=6 beakers per condition, and with a range of 25, 26, 27, 28, 29, 30 ppt salinity. Please note that each experiment was normalized separately.
 
Contributor(s) Sutherland BJ, Jantzen SG, Yasuike M, Sanderson DS, Koop BF, Jones SR
Citation(s) 23094868
Submission date May 14, 2012
Last update date Jul 02, 2014
Contact name Ben F Koop
E-mail(s) bkoop@uvic.ca
Phone (250) 472-4067
Organization name The University of Victoria
Department Biology
Lab Centre for Biomedical Research
Street address PO Box 3020 STN CSC
City Victoria
State/province BC
ZIP/Postal code V8W 3N5
Country Canada
 
Platforms (1)
GPL15566 GiLS Lepeophtheirus salmonis
Samples (78)
GSM933189 LR_S10-13 LR_SAL_Lsal_S10ppt_Cop_Trial2
GSM933190 LR_S10-14 LR_SAL_Lsal_S10ppt_Cop_Trial2
GSM933191 LR_S10-15 LR_SAL_Lsal_S10ppt_Cop_Trial2
Relations
BioProject PRJNA167141

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37976_RAW.tar 2.7 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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