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| Status |
Public on Mar 22, 2012 |
| Title |
Expression data from st 12-13 embryos from 2xbcd and 6xbcd mothers |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Embryo from 6xbcd mother shows expansion of prospective head region. In this embryo, excessive cell death can be observed. This array expreriment is for identifying differentially expressed genes in the 2xbcd and 6xbcd conditions at stage 12-13
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| Overall design |
We crossed TT16 males with TT16 females for the 2xbcd condition and bcd+t8 females for 6xbcd. In both crosses, eggs were collected in 90 minutes and incubated for 8 hours at 25°. After excluding unfertilized eggs under stereomicroscope, we extracted RNA from more than 100 eggs and hybridized by GeneChip Drosophila Genome 2.0 arrays (Affymetrix) for hybridization. We carried out paired (2xbcd vs. 6xbcd) microarray experiments in three replicates using independent RNA samples.
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| Contributor(s) |
Tanaka KM, Takahashi A |
| Citation(s) |
24671768 |
| Submission date |
Mar 21, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kentaro M Tanaka |
| E-mail(s) |
kentanak@hotmail.co.jp
|
| Organization name |
NIG
|
| Street address |
1111
|
| City |
Mishima |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| GSM898464 |
embryos from 6xbcd mother, biological rep1 |
| GSM898465 |
embryos from 2xbcd mother (control), biological rep1 |
| GSM898466 |
embryos from 6xbcd mother, biological rep2 |
| GSM898467 |
embryos from 2xbcd mother (control), biological rep2 |
| GSM898468 |
embryos from 6xbcd mother, biological rep3 |
| GSM898469 |
embryos from 2xbcd mother (control), biological rep3 |
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| Relations |
| BioProject |
PRJNA153579 |
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