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| Status |
Public on Nov 24, 2005 |
| Title |
Expression analyses to identify genes involved in testis-specific vascular development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
One of the earliest morphological changes during testicular differentiation is the establishment of an XY specific vasculature. The testis vascular system is derived from mesonephric endothelial cells that migrate into the gonad. In the XX gonad, mesonephric cell migration and testis vascular development is inhibited by WNT4 signaling. In Wnt4
mutant XX gonads, endothelial cells migrate from the mesonephros and form a male-like coelomic vessel. Consequently, the XX Wnt4 mutant mice presented an opportunity to focus a
gene expression screen on the processes of mesonephric cell migration and testicular vascular development. We compared differences in gene expression between XY Wnt4+/+ and XX Wnt4+/+ gonads and between XX Wnt4-/- and XX Wnt4+/+ gonads to identify genes similarly upregulated in wildtype XY gonads and XX mutant gonads that might play important roles in testis-specific vascular development.
Keywords: genetic sex and phenotypic comparisons
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| Overall design |
Expression profiling was performed on gonads collected from XY Wnt4+/+, XX Wnt4+/+ and Wnt4-/- embryos at 12.0 days post coitum, using arrays spotted with the Operon Mouse Genome Oligo Set, version 2.0. Competitive dual-color hybridizations with dye-swap controls were performed in duplicate for each of two comparisons: XY Wnt4+/+, versus XX Wnt4+/+, and XX Wnt4-/- versus XX Wnt4+/+. For both comparisons, the wild-type XX sample is used as the reference.
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| Contributor(s) |
Coveney D, Ross AJ, Capel B |
| Citation(s) |
16844427 |
| Submission date |
Nov 22, 2005 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Douglas Coveney |
| Organization name |
Duke University Medical Center
|
| Department |
Cell Biology
|
| Street address |
Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL3108 |
Duke University Microarray Facility Operon Mouse Oligo set, version 2.0 |
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| Samples (4)
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| GSM84973 |
Male wild-type (Cy5) versus female wild-type (Cy3) |
| GSM84974 |
Male wild-type (Cy3) versus female wild-type (Cy5) |
| GSM84975 |
Female Wnt4 -/- (Cy5) versus female Wnt4 +/+ (Cy3) |
| GSM84976 |
Female Wnt4 -/- (Cy3) versus female Wnt4 +/+ (Cy5) |
|
| Relations |
| BioProject |
PRJNA93775 |
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