|
| Status |
Public on Sep 04, 2012 |
| Title |
UTX regulated genes in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
UTX gene is localized on the X chromosome, identified as a demethylase on histone H3 lysine 27.
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| |
|
| Overall design |
Two independent UTX conditional knockout (cKO) embryonic stem cell lines and two UTX knockout (KO) cell lines were cultured on 0.1% gelatin-coated dishes without feeder cells at desity of 1 million cells/10 cm dish. Cells were cultured overnight and collected. Total RNAs were sequentially purified with Trizol (Invitrogen) and RNeasy kit (Qiagen) and analyzed on Mouse Genome 430 2.0 Array (Affymetrix) at NIDDK Microarray Core Facility following standard protocols.
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| |
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| Contributor(s) |
Wang C, Ge K |
| Citation(s) |
22949634 |
| Submission date |
Jan 30, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Chaochen Wang |
| Organization name |
NIDDK
|
| Department |
LGP
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (4)
|
| GSM867832 |
UTX conditional knockout (cKO) cell line 1 |
| GSM867833 |
UTX conditional knockout (cKO) cell line 2 |
| GSM867834 |
UTX knockout (KO) cell line 1 |
|
| Relations |
| BioProject |
PRJNA152573 |
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