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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 02, 2012 |
| Title |
Stem cell factor programs the mast cell activation phenotype |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mast cells, activated by antigen via the high affinity receptor for IgE (FcεRI), release an array of pro-inflammatory mediators that contribute to allergic disorders such as asthma and anaphylaxis. The KIT ligand, stem cell factor (SCF), is critical for mast cell expansion, differentiation and survival, and, under acute conditions, enhances mast cell activation. However, extended SCF exposure in vivo conversely protects against fatal antigen-mediated anaphylaxis. In investigating this dichotomy, we identified a novel mode of regulation of the mast cell activation phenotype through SCF-mediated programming. We found that mouse bone marrow-derived mast cells chronically exposed to SCF displayed a marked attenuation of FcεRI-mediated degranulation and cytokine production. The hypo-responsive phenotype was not a consequence of altered signals regulating calcium flux or protein kinase C, but of ineffective cytoskeletal reorganization, with evidence implicating a down-regulation of expression of the Src kinase Hck. Collectively, these findings demonstrate a major role for SCF in the homeostatic control of mast cell activation with potential relevance to mast cell-driven disease and the development of novel approaches for the treatment of allergic disorders.
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| Overall design |
Mouse bone marrow-derived mast cells were treated with IL3 or IL3+SCF. Six replicates each.
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| Contributor(s) |
Ito T, Smrž D, Jung M, Smržová Š, Bandara G, Desai A, Kuehn HS, Beaven MA, Metcalfe DD, Gilfillan AM |
| Citation(s) |
22529299 |
| Submission date |
Jan 25, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
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| Phone |
4063639248
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| Organization name |
NIH
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| Department |
NIAID
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| Lab |
RTS
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| Street address |
903 S 4th street
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| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM866205 |
Mouse bone marrow-derived mast cells treated with IL3, rep 1 |
| GSM866206 |
Mouse bone marrow-derived mast cells treated with IL3, rep 2 |
| GSM866207 |
Mouse bone marrow-derived mast cells treated with IL3, rep 3 |
| GSM866208 |
Mouse bone marrow-derived mast cells treated with IL3, rep 4 |
| GSM866209 |
Mouse bone marrow-derived mast cells treated with IL3, rep 5 |
| GSM866210 |
Mouse bone marrow-derived mast cells treated with IL3, rep 6 |
| GSM866217 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 1 |
| GSM866218 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 2 |
| GSM866219 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 3 |
| GSM866220 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 4 |
| GSM866221 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 5 |
| GSM866222 |
Mouse bone marrow-derived mast cells treated with IL3+SCF, rep 6 |
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| Relations |
| BioProject |
PRJNA152645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35332_RAW.tar |
49.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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