|
| Status |
Public on Dec 19, 2005 |
| Title |
The role of micro-RNA genes in papillary thyroid carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We show that numerous miRNAs are transcriptionally up-regulated in papillary thyroid carcinoma (PTC) tumors compared with unaffected thyroid tissue. Among the predicted target genes of the three most upregulated miRNAs (miRs 221, 222 and 146b), only less than 15% showed significant downexpression in transcript level between tumor and unaffected tissue. The KIT gene which is known to be downregulated by miRNAs 221 and 222 displayed dramatic loss of transcript and protein in those tumors that had abundant mir-221, mir-222, and mir-146b transcript.
Keywords: Disease state analysis
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| |
|
| Overall design |
Total RNA was extracted from paired tumor and normal thyroid tissues from 9 PTC patients. The same set samples were applied to Custom miRNA microarray chips (OSU_CCC version 2.0) and Affymetrix HG-U133 plus 2 chips.
|
| |
|
| Citation(s) |
16365291 |
| Submission date |
Oct 18, 2005 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sandya Liyanarachchi |
| Organization name |
OSU
|
| Department |
Human Genetics
|
| Lab |
de la Chapelle Lab
|
| Street address |
Rm 830, 460, W 12th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (18)
|
| GSM77362 |
Thyroid_ unaffected_paired with PTC14T |
| GSM77363 |
Thyroid_papillary carcinoma 14 |
| GSM77364 |
Thyroid_ unaffected_paired with PTC26T |
| GSM77365 |
Thyroid_papillary carcinoma 26 |
| GSM77366 |
Thyroid_ unaffected_paired with PTC50T |
| GSM77367 |
Thyroid_ papillary carcinoma 50 |
| GSM77368 |
Thyroid_ unaffected_paired with PTC69T |
| GSM77369 |
Thyroid_ papillary carcinoma 69 |
| GSM77370 |
Thyroid_ unaffected_paired with PTC71T |
| GSM77371 |
Thyroid_papillary carcinoma 71 |
| GSM77372 |
Thyroid_ unaffected_paired with PTC11T |
| GSM77373 |
Thyroid_ papillary carcinoma 11 |
| GSM77374 |
Thyroid_ unaffected_paired with PTC67T |
| GSM77375 |
Thyroid_papillary carcinoma 67 |
| GSM77376 |
Thyroid_ unaffected_paired with PTC77T |
| GSM77377 |
Thyroid_papillary carcinoma 77 |
| GSM77378 |
Thyroid_ unaffected_paired with PTC86T |
| GSM77379 |
Thyroid_papillary carcinoma 86 |
|
| Relations |
| BioProject |
PRJNA93411 |
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