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Series GSE34400

Query DataSets for GSE34400

Status

Public on Apr 05, 2012

Title

Buffering and proteolysis are induced upon segmental haploidy in Drosophila melanogaster.

Organism

Drosophila melanogaster

Experiment type

Expression profiling by array

Summary

Aneuploidy, i.e., variation in the number of individual chromosomes (chromosomal aneuploidy) or chromosome segment (segmental aneuploidy) is associated with developmental abnormalities and reduced fitness in all species examined, is the leading cause of miscarriages and mental retardations and a hallmark of cancer. Despite their documented importance in disease the effects of aneuploidies on the transcriptome remains largely unknown. Here we have examined the expression output in seven deficiency heterozygotes as single deficiencies and in all pairwise combinations. The results show that genes in one copy are buffered, i.e., are expressed above the expected 50% expression level compared to wild type and the buffering is general and not influenced by additional haploid regions. Long genes are significantly better buffered than short genes and our analysis suggests that gene length is the primary determinant for the degree of buffering. For short genes the degree of buffering depends on expression level and expression pattern. Furthermore, the results show that in deficiency heterozygotes the expression of genes involved in proteolysis is enhanced and negatively correlates with the degree of buffering. Our results suggest that proteolysis is a general response induced by aneuploidy.

Overall design

We prepared total RNA from flies heterozygous for seven different deletions, Df(3R)ED10953, Df(2L)ED4559, Df(2R)ED1770, Df(2R)ED1612, Df(2L)ED3, Df(3R)ED5071 or Df(3R)ED7665 in two or three single biological replicates or in pairwise combinations, as well as from six biological replicates of wild type control flies.

Contributor(s)

Lundberg LE, Figueiredo ML, Stenberg P, Larsson J

Citation(s)

Submission date

Dec 13, 2011

Last update date

Aug 28, 2018

Contact name

Jan Larsson

E-mail(s)

jan.larsson@molbiol.umu.se

Organization name

Umea University

Department

Molecular Biology

Lab

Jan Larsson

Street address

Umea University

City

Umea

ZIP/Postal code

SE-90187

Country

Sweden

Platforms (1)

[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array

Samples (44)

More...

GSM848319	Adult_female_wild_type_biological_rep_1
GSM848320	Adult_female_wild_type_biological_rep_2
GSM848321	Adult_female_wild_type_biological_rep_3

GSM848322	Adult_female_wild_type_biological_rep_4
GSM848323	Adult_female_wild_type_biological_rep_5
GSM848324	Adult_female_wild_type_biological_rep_6
GSM848325	Adult_female_Df(3R)ED10953/+_biological_rep_1
GSM848326	Adult_female_Df(2L)ED4559/+_biological_rep_1
GSM848327	Adult_female_Df(2R)ED1770/+_biological_rep_1
GSM848328	Adult_female_Df(2R)ED1612/+_biological_rep_1
GSM848329	Adult_female_Df(2L)ED3/+_biological_rep_1
GSM848330	Adult_female_Df(2L)ED4559/+_Df(3R)ED10953/+
GSM848331	Adult_female_Df(3R)ED10953/+_biological_rep_2
GSM848332	Adult_female_Df(2R)ED1612/+_Df(3R)ED10953/+
GSM848333	Adult_female_Df(2L)ED3/+_Df(3R)ED10953/+
GSM848334	Adult_female_Df(2L)ED4559/+_biological_rep_2
GSM848335	Adult_female_Df(2L)ED4559/+_Df(2R)ED1770/+
GSM848336	Adult_female_Df(2L)ED4559/+_Df(2R)ED1612/+
GSM848337	Adult_female_Df(2L)ED4559/Df(2L)ED3
GSM848338	Adult_female_Df(2R)ED1770/+_biological_rep_2
GSM848339	Adult_female_Df(2R)ED1612/+_biological_rep_2
GSM848340	Adult_female_Df(2L)ED3/+_biological_rep_2
GSM848341	Adult_female_Df(2R)ED1770/Df(2R)ED1612
GSM848342	Adult_female_Df(2R)ED1770/+_Df(2L)ED3/+
GSM848343	Adult_female_Df(2R)ED1612/+_Df(2L)ED3/+
GSM848344	Adult_female_Df(3R)ED5071/+_biological_rep_1
GSM848345	Adult_female_Df(3R)ED7665/+_biological_rep_1
GSM848346	Adult_female_Df(3R)ED5071/+_Df(2L)ED4559/+
GSM848347	Adult_female_Df(3R)ED5071/Df(3R)ED10953
GSM848348	Adult_female_Df(3R)ED5071/+_Df(2R)ED1770/+
GSM848349	Adult_female_Df(3R)ED5071/+_Df(2R)ED1612/+
GSM848350	Adult_female_Df(3R)ED5071/+_Df(2L)ED3/+
GSM848351	Adult_female_Df(3R)ED5071/+_biological_rep_2
GSM848352	Adult_female_Df(3R)ED7665/+_Df(2L)ED4559/+
GSM848353	Adult_female_Df(3R)ED7665/Df(3R)ED10953
GSM848354	Adult_female_Df(3R)ED7665/+_Df(2R)ED1770/+
GSM848355	Adult_female_Df(3R)ED7665/+_Df(2R)ED1612/+
GSM848356	Adult_female_Df(3R)ED7665/+_Df(2L)ED3/+
GSM848357	Adult_female_Df(3R)ED7665/+_biological_rep_2
GSM848358	Adult_female_Df(2L)ED4559/+_biological_rep_3
GSM848359	Adult_female_Df(3R)ED10953/+_biological_rep_3
GSM848360	Adult_female_Df(2R)ED1770/+_biological_rep_3
GSM848361	Adult_female_Df(2R)ED1612/+_biological_rep_3
GSM848362	Adult_female_Df(2L)ED3/+_biological_rep_3

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE34400_RAW.tar	85.1 Mb	(http)(custom)	TAR (of CEL)
Processed data included within Sample table

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