NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE33801 Query DataSets for GSE33801
Status Public on Jun 26, 2013
Title Environmental and simulation facility conditions can modulate a behavioral-driven altered gravity response of Drosophila imagoes transcriptome
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Genome-wide transcriptional profiling shows that reducing gravity levels in the International Space Station (ISS) causes important alterations in Drosophila gene expression. However, simulation experiments on ground, without space constraints, show weaker effects than space environment. A global and integrative analysis using the “gene expression dynamics inspector” (GEDI) self-organizing maps, reveals a subtle response of the transcriptome using different populations and microgravity and hypergravity simulation devices. These results suggest that, in addition to behavioural responses that can be detected also at the gene expression level, the transcriptome is finely tuned to normal gravity.
The alteration of this constant parameter on Earth can have effects on gene expression that depends both on the environmental conditions and the ground based facility used to compensate the gravity vector. Alternative and commons effects of mechanical facilities, like the Random Positioning Machine and a centrifuge, and strong magnetic field ones, like a cryogenically cooled superconductive magnet, are discussed.
 
Overall design We compare the effects over the gene expression profile of different gender/age Drosophila imagoes in 3-4 days-long experiments under altered gravity conditions into three GBF ("Ground Based Facilities" for micro/hyper- gravity simulation) using whole genome microarray platforms.

Descriptions of different GBFs ("treatments"):
LDC means "Large Diameter Centrifuge". Samples can be placed under three conditions: inside LDC (at certain g level), at the LDC rotational control and at external 1g control (outside the LDC).
RPM means "Random Positioning Machine". Samples can be placed under two conditions: inside RPM (at nearly 0g, Microgravity level) and at external 1g control (outside the RPM).
At the magnet, means INSIDE the Magnetic levitator (another GBF). Samples can be placed under four conditions: inside Magnet 0g* (at microgravity with magnetic field), inside Magnet at 1g* (internal control with magnetic field) or inside the magnet 2g* (at hypergravity with magnetic field) and at external 1g control (outside the magnet)
 
Contributor(s) Herranz R
Citation The behavioural-driven response of the Drosophila imago transcriptome to different types of modified gravity. Herranz R, Hill R J, Dijkstra C E, Eaves L, van Loon J J and Medina F J. Genomics Discovery 2013, 1:1; doi: 10.7243/2052-7993-1-1
Submission date Nov 18, 2011
Last update date May 04, 2018
Contact name Raúl Herranz
E-mail(s) r.herranz@csic.es
Phone +34918373112
Fax +34915360432
Organization name Centro Investigaciones Biológicas
Department Plant Cell Nucleolus, proliferation and microgravity
Lab Lab.205
Street address Ramiro de Maeztu 9
City Madrid
State/province Madrid
ZIP/Postal code 28040
Country Spain
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (50)
GSM836066 Drosophila Imagoes at RPM (0g) females, biological rep1
GSM836067 Drosophila Imagoes at RPM (0g) females, biological rep2
GSM836068 Drosophila Imagoes at RPM (0g) females, biological rep3
This SubSeries is part of SuperSeries:
GSE33803 Environmental and simulation facility conditions can modulate gravity response of Drosophila transcriptome
Relations
BioProject PRJNA154237

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33801_RAW.tar 97.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap