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| Status |
Public on Dec 31, 2015 |
| Title |
Human induced pluripotent stem cells derived from a sporadic Alzheimer's disease patient |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Induced pluripotent stem cell (iPSC) disease models have been generated for a number of diseases, and some have shown their potential utilization for pathological and drug toxicological evaluations. We have generated two hiPSC clones from a non-familial Alzheimer’s patient (AD-iPSCs), which expressed typical undifferentiated markers and fulfilled standard pluripotency assays. Genome-wide microarray analysis revealed that AD-iPSCs are highly similar to control hiPSCs and hESCs, albeit with some noticeable differences in several genes: DNAJC15, GRPR, NAIP and SNORD116-18. Several other biomarkers were differentially expressed in AD-iPSC clones, which were implicated in memory impairment and AD. Furthermore, well characterized familial AD-associated genes (APP, PSEN1, PSEN2) and non-familial (A2M, APOE, GAP43, MAOA, MPO, PLAT, PLAU, SORL1, SNCA) exhibited different expression patterns but were largely reset upon reprogramming into a pluripotent state. Overall, hiPSCs can be good candidates for AD modeling and may represent an in vitro alternative to studying disease mechanisms and drug discovery/toxicology studies.
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| Overall design |
Fibroblasts and two hiPSC clones from a non-familial Alzheimer’s patient (AD-iPSCs) were analyzed. For each cell sample, 2-3 biological replicates were obtained.
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| Contributor(s) |
Chung HC, Lin RC, Sidhu KS |
| Citation missing |
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| Submission date |
Aug 02, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Henry Chung |
| E-mail(s) |
henry.chung@unswalumni.com
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| Phone |
61 2 9382 2620
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| Organization name |
Prince of Wales Hospital
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| Department |
Clinical Sciences Bld
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| Lab |
Stem Cell Lab
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| Street address |
High St
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| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2031 |
| Country |
Australia |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA144799 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31163_RAW.tar |
32.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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