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| Status |
Public on Sep 29, 2011 |
| Title |
Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
In Drosophila, Piwi proteins associate with Piwi-interacting RNAs (piRNAs) and protect the germline genome by silencing mobile genetic elements. This defense system acts in germline and gonadal somatic tissue to preserve germline development. Genetic control for these silencing pathways varies greatly between tissues of the gonad. Here, we identified Vreteno (Vret), a novel gonad-specific protein essential for germline development. Vret is required for piRNA-based transposon regulation in both germline and somatic gonadal tissues. We show that Vret, which contains Tudor domains, associates physically with Piwi and Aubergine (Aub), stabilizing these proteins via a gonad-specific mechanism, absent in other fly tissues. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline, piRNAs can engage in an Aub/Argonaute 3 (AGO3)-dependent amplification in the absence of Vret, suggesting that Vret function can distinguish between primary piRNAs loaded into Piwi/Aub complexes and piRNAs engaged in the amplification cycle. We propose that Vret acts at an early step in primary piRNA processing where it plays an essential role in transposon regulation.
These studies show that vreteno (vret) has a role in germline development and primary piRNA regulation in Drosophila.
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| Overall design |
Transposable element expression profiles from Drosophila ovaries mutant for vreteno, piwi and aubergine were compared using genome-wide mRNA expression profiling by Affymetrix GeneChip arrays (Drosophila 2.0). Key targets were validated by qPCR experiments.
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| Contributor(s) |
Zamparini AL, Davis MY, Malone CD, Vieira E, Zavadil J, Sachidanandam R, Hannon GJ, Lehmann R |
| Citation(s) |
21831924 |
| Submission date |
Jul 01, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
zavadilj@iarc.fr
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| Phone |
+33648065733
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| Organization name |
IARC WHO
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| Street address |
150 COURS ALBERT THOMAS
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| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| GSM753468 |
vret heterozygous control ovaries, repeat 1 |
| GSM753469 |
vret heterozygous control ovaries, repeat 2 |
| GSM753470 |
vret homozygous mutant ovaries, repeat 1 |
| GSM753471 |
vret homozygous mutant ovaries, repeat 2 |
| GSM753472 |
piwi heterozygous control ovaries, repeat 1 |
| GSM753473 |
piwi heterozygous control ovaries, repeat 2 |
| GSM753474 |
piwi trans-heterozygous mutant ovaries, repeat 1 |
| GSM753475 |
piwi trans-heterozygous mutant ovaries, repeat 2 |
| GSM753476 |
aub heterozygous control ovaries, repeat 1 |
| GSM753477 |
aub heterozygous control ovaries, repeat 2 |
| GSM753478 |
aub trans-heterozygous mutant ovaries, repeat 1 |
| GSM753479 |
aub trans-heterozygous mutant ovaries, repeat 2 |
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| Relations |
| BioProject |
PRJNA143637 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30360_RAW.tar |
24.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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