|
| Status |
Public on Jul 12, 2011 |
| Title |
Tuberous sclerosis complex 1 (Tsc1) enforces quiescence of naive T cells to promote immune homeostasis and function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The mechanisms that regulate T cell quiescence are poorly understood. We report that tuberous sclerosis complex 1 (Tsc1) establishes a quiescent program in naïve T cells by controlling cell size, cell cycle entry, and responses to T cell receptor stimulation. Loss of quiescence predisposed Tsc1-deficient T cells to apoptosis that depleted conventional T cells and invariant natural killer T cells. Loss of Tsc1 function dampened in vivo immune responses to bacterial infection. Tsc1-deficient T cells exhibited increased mTORC1 but diminished mTORC2 activities, with mTORC1 activation essential for the disruption of immune homeostasis. Therefore, Tsc1-dependent control of mTOR is crucial in establishing naïve T cell quiescence to facilitate adaptive immune function.
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| Overall design |
Naïve CD4 and CD8 T cells from wild-type and Tsc1-deficient mice (in triplicates each group) were stimulated with or without TCR signaling. RNA was analyzed by microarrays. WT/KO for 0 and 4 hr for CD4 (triplicates), and WT/KO for 0 hr for CD8 (duplicates).
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| Contributor(s) |
Yang K, Neale G, Green DR, He W, Chi H |
| Citation(s) |
21765414 |
| Submission date |
Jun 07, 2011 |
| Last update date |
Aug 06, 2018 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (16)
|
| GSM738363 |
wild-type naïve CD4 T cells KAI264 |
| GSM738365 |
wild-type naïve CD4 T cells with CD3-CD28 stimulation for 4h KAI265 |
| GSM738367 |
wild-type naïve CD4 T cells KAI267 |
| GSM738368 |
wild-type naïve CD4 T cells with CD3-CD28 stimulation for 4h KAI268 |
| GSM738370 |
wild-type naïve CD4 T cells KAI270 |
| GSM738372 |
wild-type naïve CD4 T cells with CD3-CD28 stimulation for 4h KAI271 |
| GSM738373 |
Tsc1-deficient CD4 naïve cells KAI273 |
| GSM738375 |
Tsc1-deficient CD4 naïve cells with CD3-CD28 stimulation for 4h KAI274 |
| GSM738377 |
Tsc1-deficient CD4 naïve cells KAI276 |
| GSM738378 |
Tsc1-deficient CD4 naïve cells with CD3-CD28 stimulation for 4h KAI277 |
| GSM738379 |
Tsc1-deficient CD4 naïve cells KAI279 |
| GSM738380 |
Tsc1-deficient CD4 naïve cells with CD3-CD28 stimulation for 4h KAI280 |
| GSM738381 |
wild-type naïve CD8 T cells KAI282 |
| GSM738382 |
wild-type naïve CD8 T cells KAI284 |
| GSM738383 |
Tsc1-deficient CD8 naïve cells KAI286 |
| GSM738384 |
Tsc1-deficient CD8 naïve cells KAI288 |
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| Relations |
| BioProject |
PRJNA141013 |
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