|
| Status |
Public on Sep 30, 2011 |
| Title |
Changes in the Bone Marrow Microenvironment Impact Anabolic Actions of PTH |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Irradiation induced bone marrow ablation ultimately enhanced PTH anabolic effects in bone.
B6 mice at 10 days of age were sub-lethally irradiated and treated with PTH 24h later for 5 days. 24h post last-injection, bone marrow was flushed with Trizol and RNA isolated and purified. Microarray analyses was performed to determine differential differences in PTH effects in non-irradiated vs. irradiated bone marrow.
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| |
|
| Overall design |
Triplicates of 4 groups (total of 12 samples) which include: Nonirradiated Vehicle, Nonirradiated PTH, Irradiated Vehicle and Irradiated PTH
|
| |
|
| Contributor(s) |
McCauley LK, Koh AJ |
| Citation(s) |
22045660 |
| Submission date |
Apr 24, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Amy J Koh |
| Organization name |
University of Michigan
|
| Department |
POM
|
| Lab |
Laurie K. McCauley
|
| Street address |
1011 N. University Ave.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (12)
|
| GSM713688 |
whole bone marrow-nonirradiated-vehicle-rep1 |
| GSM713689 |
whole bone marrow-nonirradiated-vehicle-rep2 |
| GSM713690 |
whole bone marrow-nonirradiated-vehicle-rep3 |
| GSM713691 |
whole bone marrow-nonirradiated-PTH-rep1 |
| GSM713692 |
whole bone marrow-nonirradiated-PTH-rep2 |
| GSM713693 |
whole bone marrow-nonirradiated-PTH-rep3 |
| GSM713694 |
whole bone marrow-irradiated-vehicle-rep1 |
| GSM713695 |
whole bone marrow-irradiated-vehicle-rep2 |
| GSM713696 |
whole bone marrow-irradiated-vehicle-rep3 |
| GSM713697 |
whole bone marrow-irradiated-PTH-rep1 |
| GSM713698 |
whole bone marrow-irradiated-PTH-rep2 |
| GSM713699 |
whole bone marrow-irradiated-PTH-rep3 |
|
| Relations |
| BioProject |
PRJNA138773 |
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