 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 19, 2011 |
| Title |
Expression data from STAG2 deficient and proficient human cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have discovered frequent genetic inactivation of the STAG2 gene in diverse human cancers including glioblastoma, Ewing's sarcoma, and melanoma. STAG2 encodes a subunit of the sister chromatid cohesion complex called the "cohesin complex" that is responsible for the cohesion of sister chromatids following DNA replication and is cleaved at the metaphase to anaphase transition to enable chromosome segregation into daughter cells. Interestingly, the cohesin complex has also been implicated as a regulator of chromatin architecture and transcription. To determine the functional significance of STAG2 inactivation in cancer pathogenesis, we used somatic cell gene targeting to correct the endogenous mutations of STAG2 in two aneuploid human glioblastoma cell lines, H4 and 42MGBA. Similarly, somatic cell gene targeting was also used to introduce a nonsense mutation into codon 6 of the endogenous wild-type allele of STAG2 in HCT116 cells, a near-diploid human colorectal cancer cell line with stable karyotype. Expression profiling of these three paired sets of STAG2-proficient and deficient cells demonstrated that STAG2 does not play a global role in transcriptional regulation nor does it recurrently modulate the expression of specific tumor-promoting or suppressing genes.
For further details, see Solomon et al., Mutational inactivation of STAG2 causes aneuploidy in human cancer.
|
| |
|
| Overall design |
RNA was prepared from subconfluent asynchronously proliferating cells using TRIZOL and purified by RNeasy MinElute Cleanup kit (Qiagen). Hybridization to Affymetrix GeneChip Human Gene 1.0 ST arrays was used to generate gene expression profiles of STAG2-deficient and proficient H4, 42MGBA, and HCT116 cells.
|
| |
|
| Contributor(s) |
Solomon DA, Elkahloun AG, Wu W |
| Citation(s) |
21852505 |
| Submission date |
Mar 28, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
abdel G Elkahloun |
| E-mail(s) |
abdel@mail.nih.gov
|
| Phone |
301 402 3170
|
| Organization name |
NHGRI-NIH
|
| Lab |
MICROARRAY CORE
|
| Street address |
50, SOUTH DRIVE
|
| City |
BETHESDA |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (16)
|
| GSM698531 |
H4 parental cells (STAG2 mutant) |
| GSM698532 |
H4 non-recombinant clone 10 (STAG2 mutant) |
| GSM698533 |
H4 non-recombinant clone 12 (STAG2 mutant) |
| GSM698534 |
H4 STAG2 knock-in post-Cre clone 8-1 (STAG2 corrected) |
| GSM698535 |
H4 STAG2 knock-in post-Cre clone 8-3 (STAG2 corrected) |
| GSM698536 |
H4 STAG2 knock-in post-Cre clone 88-1 (STAG2 corrected) |
| GSM698537 |
42MGBA parental cells (STAG2 mutant) |
| GSM698538 |
42MGBA STAG2 knock-in pre-Cre clone 53 (STAG2 deficient) |
| GSM698539 |
42MGBA STAG2 knock-in pre-Cre clone 92 (STAG2 deficient) |
| GSM698540 |
42MGBA STAG2 knock-in post-Cre clone 53-1 (STAG2 corrected) |
| GSM698541 |
42MGBA STAG2 knock-in post-Cre clone 53-7 (STAG2 corrected) |
| GSM698542 |
42MGBA STAG2 knock-in post-Cre clone 92-6 (STAG2 corrected) |
| GSM698543 |
HCT116 non-recombinant clone 24 (STAG2 wild-type) |
| GSM698544 |
HCT116 non-recombinant clone 27 (STAG2 wild-type) |
| GSM698545 |
HCT116 STAG2 knockout clone 7 (STAG2 deficient) |
| GSM698546 |
HCT116 STAG2 knockout clone 21 (STAG2 deficient) |
|
| Relations |
| BioProject |
PRJNA139663 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28214_RAW.tar |
67.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...