|
| Status |
Public on Mar 08, 2011 |
| Title |
Zebrafish: Estrogen induced gene expression |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed microarray experiment on zebrafish exposed to estrogen (17β-estradiol; a classified carcinogen) and an anti-estrogen (ICI 182,780). Our transcriptome analysis captured multiple estrogen-responsive genes and signaling pathways that increased cell proliferation, promoted DNA damage and genome instability, and decreased tumor suppressing effects, suggesting a common mechanism for estrogen-induced carcinogenesis. Comparative analysis with human E2 responsive genes revealed a core set of conserved estrogen-responsive genes that demonstrate enrichment of ER binding sites and cell cycle signaling pathways. Knowledge-based and network analysis led us to propose that the mechanism involving estrogen-activated ER mediated down-regulation certain genes followed by up-regulation cell cycle-related genes is highly conserved. Our study provides first evidence of molecular conservation of estrogen-responsiveness between zebrafish and human cancer cell lines, hence demonstrating the potential of zebrafish for estrogen-related cancer research.
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| |
|
| Overall design |
10 samples were analyzed in total. Three samples in control, 3 samples treated with estrogen and 3 samples treated with estrogen in combination with ICI.
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| |
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| Contributor(s) |
Mathavan S, Lee SG, Lam SH, Murthy KR, Liu ET, Gong Z |
| Citation(s) |
21575170 |
| Submission date |
Mar 07, 2011 |
| Last update date |
Mar 09, 2020 |
| Contact name |
Justin Jeyakani Joseph Gnanakkan |
| E-mail(s) |
gnanakkan@gis.a-star.edu.sg
|
| Phone |
68088173
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Computational and Systems Biology
|
| Street address |
60, Biopolis street
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
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| Platforms (1) |
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| Samples (10)
|
| GSM686149 |
Zebrafish control 1 (ethanol control 0.01%v/v) |
| GSM686150 |
Zebrafish control 2 (ethanol control 0.01%v/v) |
| GSM686151 |
Zebrafish control 3 (ethanol control 0.01%v/v) |
| GSM686152 |
Zebrafish control 4 (ethanol control 0.01%v/v) |
| GSM686153 |
Zebrafish exposed to Estrogen 1 (17b-estradiol; 10mM final concentration) |
| GSM686154 |
Zebrafish exposed to Estrogen 2 (17b-estradiol; 10mM final concentration) |
| GSM686155 |
Zebrafish exposed to Estrogen 3 (17b-estradiol; 10mM final concentration) |
| GSM686156 |
Zebrafish exposed to Estrogen 1 (17b-estradiol; 10mM final concentration and ICI 1uM final concentration) |
| GSM686157 |
Zebrafish exposed to Estrogen 2 (17b-estradiol; 10mM final concentration and ICI 1uM final concentration) |
| GSM686158 |
Zebrafish exposed to Estrogen 3 (17b-estradiol; 10mM final concentration and ICI 1uM final concentration) |
|
| Relations |
| BioProject |
PRJNA138129 |
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