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| Status |
Public on Jan 29, 2011 |
| Title |
Ku70 Is a Novel Cytosolic DNA Sensor that Induces Type-III Rather than Type-I IFN |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cytosolic foreign DNA is detected by pattern recognition receptors and mainly induces Type-I IFN production. We found that transfection of different types of DNA into various untreated cells induces Type-III IFN (IFN-lambda1) rather than Type-I IFN, indicating the presence of uncharacterized DNA sensor(s). A pull-down assay using cytosolic proteins identified that Ku70 and Ku80 are the DNA binding proteins. The knockdown studies and the reporter assay revealed that Ku70 is a novel DNA sensor inducing the IFN-lambda1 activation. The functional analysis of IFNL1 promoter revealed that PRDI and ISRE sites are predominantly involved in the DNA-mediated IFNL1 activation. A pull-down assay using nuclear proteins demonstrated that the IFN-lambda1 induction is associated with the activation of IRF-1 and IRF-7. This is the first report of a specific induction of Type-III rather than Type-I IFN and of Ku70 that plays a key role in the activation of innate immune responses.
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| Overall design |
To identify the nature of the anti-HIV mediators associated with the empty vector transfection, we compared patterns of gene expression between untreated and pCMV9-transfected HEK293 cells, using DNA microarray analysis.
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| Contributor(s) |
Zhang X, Brann TW, Zhou M, Yang J, Oguariri RM, Lidie KB, Imamichi H, Huang D, Lempicki RA, Baseler MW, Veenstra TD, Young HA, Lane HC, Imamichi T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 28, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Richard A Lempicki |
| E-mail(s) |
rlempicki@mail.nih.gov
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| Phone |
301-846-5093
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| Organization name |
Leidos Biomedical Research, Inc.
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| Department |
Clinical Services Program
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| Lab |
Laboratory of Immunopathogenesis and Bioinformatics
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| Street address |
PO Box B
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| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA136943 |
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