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| Status |
Public on May 16, 2024 |
| Title |
Unveiling the Immune Dynamics of Neisseria Persistent Oral Colonization |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Commensal bacteria are crucial in maintaining host physiological homeostasis, immune system development, and protection against pathogens. Despite their significance, the factors influencing persistent bacterial colonization and their impact on the host still need to be fully understood. Animal models have served as valuable tools to investigate these interactions, but most have limitations. The bacterial genus Neisseria, which includes both commensal and pathogenic species, has been studied from a pathogenicity to humans’ perspective, but lacks models that study immune responses in the context of long-term persistence. Neisseria musculi, a recently described natural commensal of mice, offers a unique opportunity to study long-term host-commensal interactions. In this study, for the first time we have used this model to study the transcriptional, phenotypic, and functional dynamics of immune cell signatures in the mucosal and systemic tissue of mice in response to Neisseria musculi colonization. We found key genes and pathways vital for immune homeostasis in palate tissue, validated by flow cytometry of immune cells from lung, blood and spleen. This study offers a novel avenue for advancing our understanding of host-bacteria dynamics and may provide a platform for developing efficacious interventions against mucosal persistence by pathogenic Neisseria.
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| Overall design |
Investigation of immune responses to Neisseria musculi colonization in a novel AJ mouse model. Hard palate samples were harvested 5 or 33 days post inoculation or mock inoculation, and used for total RNA extraction and RNAseq.
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| Contributor(s) |
Alles M, Gunasena M, Zia T, D’Mello A, Bhattarai S, Mulhern W, Terry L, Scherger T, Wijeratne S, Singh S, Wijeratne AJ, Kasturiratna D, Tettelin H, Weyand NJ, Liyanage NP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 15, 2024 |
| Last update date |
May 16, 2024 |
| Contact name |
Suvarna Nadendla |
| Organization name |
University of Maryland School of Medicine
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| Department |
Institute for Genome Sciences
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| Street address |
670 W.Baltimore Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1111780 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267528_Neisseria_musculi_AJ_mouse_model_InfectImmun2024_raw_counts.txt.gz |
907.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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