|
| Status |
Public on May 14, 2024 |
| Title |
FOXA1 and FOXA2 binding profile in mouse colon [ChIP] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
FOXA1 and FOXA2 are essentail transcription factors for proper gut development. In adults, they play a role in the differentiation of intestinal secretory cells and were also reporeted to directly activate Muc2 transcription. Here we show that FOXA1 and FOXA2 bind near goblet cell genes, and are specifically enriched near glycosylation genes. Deletion of these transcription factors in mouse intestine leads to a downregulation of glycosylation genes in the colon and to a massive change of the colonic surface glycans. I turn, the microbiome composition shifts dramatically and spontaneous inflammatory bowel disease ensued. We conclude that vertebrates shape a favorable microbiome by establishing a glycocalyx to nurture specific bacterial taxa through control of the epithelial glycosylation program by the FoxA transcription factors.
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| |
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| Overall design |
ChIP-seq for FOXA1 and FOXA2 on isolated colonic crypts from wild type C57BL/6 male mice.
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| |
|
| Contributor(s) |
Kieckhaefer J, Swisa A, Kaestner KH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA1091956 |
| Submission date |
Apr 10, 2024 |
| Last update date |
May 14, 2024 |
| Contact name |
Avital Swisa |
| E-mail(s) |
avital.swisa@mail.huji.ac.il
|
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Civic Center Blvd
|
| City |
PHILADELPHIA |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
| GSM8195643 |
FOXA1_ChIP-seq of mus musculus: colonic crypts_rep1 |
| GSM8195644 |
FOXA1_ChIP-seq of mus musculus: colonic crypts_rep2 |
| GSM8195645 |
FOXA1_ChIP-seq of mus musculus: colonic crypts_rep3 |
| GSM8195646 |
FOXA1_ChIP-seq of mus musculus: colonic crypts_rep4 |
| GSM8195647 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep1 |
| GSM8195648 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep2 |
| GSM8195649 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep3 |
| GSM8195650 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep4 |
| GSM8195651 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep5 |
| GSM8195652 |
FOXA2_ChIP-seq of mus musculus: colonic crypts_rep6 |
| GSM8195653 |
Input_ChIP-seq of mus musculus: colonic crypts_rep1 |
| GSM8195654 |
Input_ChIP-seq of mus musculus: colonic crypts_rep2 |
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