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| Status |
Public on Apr 25, 2024 |
| Title |
Gene expression profile of in-vitro differentiated Dendritic Cells (DCs) from induced pluripotent stem cell (iPSC) and cord blood sources |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dendritic cells (DCs) are rare innate immune cells that are essential regulators of anti-tumour, anti-viral and vaccine responses by the adaptive immune system. We established a protocol to differentiate human dendritic cells from iPSCs and compare their behaviour with cord blood_derived equivalents. We used bulk RNA-seq analysis to characterise the different subsets of in vitro-generated DCs including DC1, DC2A and DC2B.
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| Overall design |
In vitro-differentiated DC subsets from iPSC and cord blood CD34+ progenitors were isolated by Fluorescence-activated cell sorting (FACS) as Live HLADR+ CD11c+ CD141+ CLEC9A+ DC1, HLADR+ CD11c+ CD1c+ CD14- DC2A and HLADR+ CD11c+ CD1c+ CD14+ DC2B subsets and were analysed by bulk RNA sequencing.
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| Contributor(s) |
Elahi Z, Wells CA, Butcher S |
| Citation missing |
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| Submission date |
Mar 15, 2024 |
| Last update date |
Apr 25, 2024 |
| Contact name |
Stemformatics . |
| Organization name |
The University of Melbourne
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| Street address |
30 Royal Parade
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| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA1088507 |
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