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| Status |
Public on May 01, 2024 |
| Title |
The gene expression of HUVEC in response to the cytokine stimulations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The response of endothelial cells to the tissue environment has a critical impact on their function in inflammation and tumors. In particular, cytokine stimulation such as TNFa, IL-4, IFNg, and LTbR induces gene expression changes, which significantly impact endothelial cell function and differentiation. To understand the degree of gene expression change, we performed a transcriptome analysis of HUVEC.
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| Overall design |
HUVEC cells were cultured at 2X10(5)/well in the 6-well plate. The next day, the cell medium was replaced with 10 ng/ml TNFa, 10 ng/ml IL-4, 50 ng/ml IFNg, or control (EGM-2 medium only) and cultured 2 days. RNA was isolated and used for the library generation.
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| Contributor(s) |
Junko S, Masanobu K, Lu J |
| Citation(s) |
38402584 |
| Submission date |
Feb 02, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
JUNKO SAWADA |
| E-mail(s) |
jsawada1@jhmi.edu
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| Organization name |
Johns Hopkins All Children's Hispital
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| Street address |
600 5th Street South
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| City |
St. Petersburg |
| ZIP/Postal code |
33701 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| GSM8061356 |
HUVEC, TNFa1 |
| GSM8061357 |
HUVEC, TNFa2 |
| GSM8061358 |
HUVEC, TNFa3 |
| GSM8061359 |
HUVEC,LTa1b2-1 |
| GSM8061360 |
HUVEC,LTa1b2-2 |
| GSM8061361 |
HUVEC,LTa1b2-3 |
| GSM8061362 |
HUVEC, IL4-1 |
| GSM8061363 |
HUVEC, IL4-2 |
| GSM8061364 |
HUVEC, IL4-3 |
| GSM8061365 |
HUVEC, IFNg-1 |
| GSM8061366 |
HUVEC, IFNg-2 |
| GSM8061367 |
HUVEC, IFNg-3 |
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| Relations |
| BioProject |
PRJNA1072704 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE254958_FPKM.xlsx |
3.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
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