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| Status |
Public on Apr 23, 2024 |
| Title |
Multiomic profiling of medulloblastoma reveals subtype-specific targetable alterations at the proteome and N-glycan level [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Medulloblastomas (MBs) are malignant pediatric brain tumors that are molecularly and clinically very heterogenous. To unravel phenotypically relevant MB subtypes, we compiled a harmonized proteome dataset of 167 MBs and integrated findings with DNA methylation and N-glycome data. Six proteome MB subtypes emerged, that could be assigned to two main molecular programs: transcription/translation (pSHHt, pWNT and pGroup3-Myc), and synapses/immunological processes (pSHHs, pGroup3 and pGroup4). Multiomic analysis revealed different conservation levels of proteome features across MB subtypes at the DNA-methylation level. Aggressive pGroup3-Myc MBs and favorable pWNT MBs were most similar in cluster hierarchies concerning overall proteome patterns but showed different protein abundances of the vincristine resistance associated multiprotein complex TriC/CCT and of N-glycan turnover associated factors. The N-glycome reflected proteome subtypes and complex-bisecting N-glycans characterized pGroup3-Myc tumors. Our results shed light on new targetable alterations in MB and set a foundation for potential immunotherapies targeting glycan structures
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| Overall design |
21 human MB samples were analyzed using RNA Seq. Data was integrated with DNA Methylome and Proteome data
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| Contributor(s) |
Godbole S, Voss H, Neumann JE |
| Citation missing |
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| Submission date |
Sep 22, 2023 |
| Last update date |
Apr 23, 2024 |
| Contact name |
Julia Elisabeth Neumann |
| Organization name |
University Hospital Hamburg Eppendorf
|
| Department |
ZMNH
|
| Street address |
Martinistrasse 52
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE243796 |
Multiomic profiling of medulloblastoma reveals subtype-specific targetable alterations at the proteome and N-glycan level |
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| Relations |
| BioProject |
PRJNA1020067 |
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