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| Status |
Public on May 07, 2024 |
| Title |
Active mRNA transcripts obtained using ribosomal pulldown from mouse myocytes (myofibers) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We have generated mice expressing a hemagglutinin tag in the ribosomes of myocytes (Myogenin-Cre x RiboTag-Rpl22fl/fl-HA) to be able to isolate active (polysome-bound) myocyte transcripts under various experimental factors (e.g. sex, age, exercise status)
RNA was obtained by immunoprecipitation of HA-tagged polysomes of left leg solei
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| Overall design |
5 wt control samples (sedentary) were analyzed to see the relative levels of myocyte expressed genes. This is a first release of a broader study on the effects of various experimental factors (e.g. sex, age, exercise status) on the active gene expression of myocytes
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| Contributor(s) |
Serrano J, Kyriazis G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2023 |
| Last update date |
May 08, 2024 |
| Contact name |
George Kyriazis |
| E-mail(s) |
kyriazis.2@osu.edu
|
| Organization name |
The Ohio State University
|
| Street address |
460 W 12th Ave
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43202 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1010201 |
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