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| Status |
Public on Apr 19, 2024 |
| Title |
Caspase-mediated processing of TRBP regulates apoptosis during viral infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RNA silencing is a post-transcriptional gene-silencing mechanism mediated by microRNAs (miRNAs). However, the regulatory mechanism of RNA silencing during viral infection is unclear. TAR RNA-binding protein (TRBP) is an enhancer of RNA silencing that induces miRNA maturation by interacting with the ribonuclease Dicer. TRBP interacts with a virus sensor protein, laboratory of genetics and physiology 2 (LGP2), in the early stage of viral infection of human cells. Next, it induces apoptosis by inhibiting the maturation of miRNAs, thereby upregulating the expression of apoptosis regulatory genes. In this study, we show that TRBP undergoes a functional conversion in the late stage of viral infection. Viral infection resulted in the activation of caspases that proteolytically processed TRBP into two fragments. The N-terminal fragment did not interact with Dicer but interacted with type I interferon (IFN) signaling modulators, such as protein kinase R (PKR) and LGP2, and induced ER stress. The end results were irreversible apoptosis and suppression of IFN signaling. Our results demonstrate that the processing of TRBP enhances apoptosis, reducing IFN signaling during viral infection.
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| Overall design |
Total RNAs were extracted from mock-treated or SeV infected TRBP knockout cells expressing TRBP-WT or -D234A and performed microarray analysis.
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| Contributor(s) |
Ui-Tei K, Takahashi T |
| Citation(s) |
38636948 |
| Submission date |
Jun 09, 2023 |
| Last update date |
Jun 06, 2024 |
| Contact name |
Tomoko Takahashi |
| E-mail(s) |
takahas@mail.saitama-u.ac.jp
|
| Organization name |
The University of Tokyo
|
| Lab |
Ui-Tei lab
|
| Street address |
2-11-16 Yayoi
|
| City |
Bunkyo-ku, Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
|
| Samples (4)
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| GSM7470617 |
TRBP KO cervical cancer cells, mock_WT |
| GSM7470618 |
TRBP KO cervical cancer cells, SeV_WT |
| GSM7470619 |
TRBP KO cervical cancer cells, mock_D234A |
| GSM7470620 |
TRBP KO cervical cancer cells, SeV_D234A |
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| This SubSeries is part of SuperSeries: |
| GSE234550 |
Caspase-mediated processing of TRBP regulates apoptosis during viral infection |
|
| Relations |
| BioProject |
PRJNA982032 |
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