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| Status |
Public on Aug 24, 2010 |
| Title |
Genome-wide analysis of time-dependent gene expression in MCF-10A cells treated with the EGFR-specific inhibitor gefitinib for 45 hours |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Results of blocking EGFR kinase activity in MCF-10A cells by treatment with gefitinib and measuring gene expression as a function of time provides information as to what genes are regulated by EGFR in these nontransformed breast epithelial cells.
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| Overall design |
MCF-10A cells were treated with the EGFR-specific small molecule kinase inhibitor gefitinib for 45 hours. Total RNA was collected every 3 hours from parallel cultures throughout the 45 hour duration of treatment and genome-wide analysis of expression was performed on RNA from each time point (total 16 time points, starting at 0 hours treatment).
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| Contributor(s) |
Bollig-Fischer A, Ethier SP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 25, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Aliccia B Bollig-Fischer |
| E-mail(s) |
bollig@karmanos.org
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| Organization name |
Karmanos Cancer Inst., Wayne State University
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| Street address |
4100 John R, HWCRC rm 815
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| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
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| Platforms (1) |
| GPL6255 |
Illumina humanRef-8 v2.0 expression beadchip |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE23175 |
HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation |
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| Relations |
| BioProject |
PRJNA133237 |
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