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| Status |
Public on Jul 24, 2010 |
| Title |
Expression data from Hodgkin lymphoma cell lines UHO-1 and L-1236 transduced with shRNAs against GATA-3 or non-functional control shRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factor network in Hodgkin lymphoma (HL) represents a unique composition of proteins found in no other hematopoietic cell. Among these factors, an aberrant expression of the T cell transcription factor GATA-3 is observed in the B cell-derived Hodgkin and Reed/Sternberg (HRS) tumor cells. Herein, we elucidated the regulation and function of this factor in HL
To obtain a more comprehensive overview of GATA-3 regulated genes in cHL cell lines, we performed a gene chip analysis comparing gene expression in GATA-3-specific shRNA-transduced L-1236 and U-HO1 cells compared to samples transduced with vectors encoding scrambled shRNAs.
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| Overall design |
12 Total samples were analyzed. Three independent samples were analyzed per condition and cell line.We generated the following pairwise comparisons using Bioconductor: shRNA Gata-3 UHO-1 < control shRNA UHO-1; shRNA Gata-3 L1236 < control shRNA L-1236. Genes with an FDR≤ 25% and a fold-change ≥ 1.5 and ≤ -1.5 were selected.
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| Contributor(s) |
Stanelle J, Döring C |
| Citation(s) |
20660789 |
| Submission date |
Jun 24, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Claudia Döring |
| E-mail(s) |
C.Doering@em.uni-frankfurt.de
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| Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
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| Department |
Medical School, Institite of Pathology
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| Street address |
Theodor Stern Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA128179 |
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