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| Status |
Public on Jul 15, 2022 |
| Title |
Molecular Neurobiology of Loss Part 2 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Psychological loss is a common experience that erodes well-being and negatively impacts quality of life. The molecular underpinnings of loss are poorly understood, making it challenging to develop treatment strategies. Here, we investigate the mechanisms of loss using an enrichment removal (ER) paradigm in rats. RNA-seq was used to investigate the molecular landscape of the basolateral amygdala (BLA), a region previously implicated in loss, in adult male Sprague-Dawley rats following ER. Rats were subjected to standard housing (SH) for 6 weeks, environmental enrichment (EE) for 6 weeks, or enrichment removal (ER), consisting of 4 weeks of EE followed by 2 weeks of removal into impoverished conditions (n=10/pool/group). Rats were then perfused and micropunches were collected from the basolateral amygdala (BLA) for bulk RNA-seq.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data collected from the basolateral amygdala of rats subjected to standard housing conditions, environmental enrichment, or enrichment removal (10 rats pooled into 1 biological replicate/group).
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| Contributor(s) |
Smail MA, Herman JP, McCullumsmith RE, Shukla R |
| Citation(s) |
37644175 |
| Submission date |
Jul 12, 2022 |
| Last update date |
Mar 13, 2024 |
| Contact name |
Marissa Ann Smail |
| E-mail(s) |
smailma@mail.uc.edu
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| Phone |
7242167360
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| Organization name |
University of Cincinnati
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| Department |
Pharmacology and Systems Physiology
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| Lab |
Herman
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| Street address |
4227 Allendorf Drive, Apt 1
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| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45209 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA858032 |
