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| Status |
Public on Feb 17, 2011 |
| Title |
mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7
Keywords: Hepatocellular carcinoma, Expression array, microRNA
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| Overall design |
microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.
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| Contributor(s) |
Toffanin S, Hoshida Y, Lachenmayer A, Villanueva A, Cabellos L, Minguez B, Ward S, Thung S, Savic R, Chiang DY, Armengol CA, Tovar V, Roayaie S, April C, Fan J, Schwartz M, Bruix J, Friedman SL, Golub TR, Mazzaferro V, Llovet JM |
| Citation(s) |
21324318 |
| Submission date |
Mar 08, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM518632 |
Human hepatocellular carcinoma cell line infected with GFP protein (control) 1 |
| GSM518633 |
Human hepatocellular carcinoma cell line infected with GFP protein (control) 2 |
| GSM518634 |
Human hepatocellular carcinoma cell line infected with GFP protein (control) 3 |
| GSM518635 |
Human hepatocellular carcinoma cell line infected with MIR517A and GFP protein 1 |
| GSM518636 |
Human hepatocellular carcinoma cell line infected with MIR517A and GFP protein 2 |
| GSM518637 |
Human hepatocellular carcinoma cell line infected with MIR517A and GFP protein 3 |
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| This SubSeries is part of SuperSeries: |
| GSE20596 |
MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a |
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| Relations |
| BioProject |
PRJNA129591 |
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