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| Status |
Public on May 24, 2023 |
| Title |
RNAseq of cultured neonatal rat ventricular cardiomyocytes treated with siRNA for Btg1 and Btg2 knockdown. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Total RNA was isolated from n=3 culture wells per siRNA treatment group, from 6 independent primary neonatal rat cardiomyocyte isolations. RNA was pooled to obtain n=3 independent samples per siRNA treatment group for sequencing. The siRNA treatment groups were= siScr (Scramble control), siBtg12 (Btg1/Btg2 double knockdown), siBtg1 (Btg1 single knockdown), siBtg2 (Btg2 single knockdown).
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| Overall design |
Total RNA from siRNA-mediated Btg1 and Btg2-depleted cultured neonatal rat cardiomyocytes were used for RNAseq.
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| |
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| Contributor(s) |
Velayutham N, Yutzey KE |
| Citation(s) |
37062247 |
| Submission date |
May 20, 2022 |
| Last update date |
May 24, 2023 |
| Contact name |
Katherine E Yutzey |
| E-mail(s) |
katherine.yutzey@cchmc.org
|
| Phone |
+1 5136365958
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Molecular Cardiovascular Biology
|
| Lab |
Yutzey
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA840946 |
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