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| Status |
Public on Mar 28, 2011 |
| Title |
Stabilization of ß-catenin during murine Kidney Development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We define a pathogenic role for a ß-catenin-activated genetic pathway in murine renal dysplasia. Cre-mediated stabilization of ß-catenin in the ureteric cell lineage prior to the onset of kidney development increased ß-catenin levels and caused renal aplasia or severe hypodysplasia. A genome-wide analysis of mRNA expression in dysplastic tissue identified down-regulation of genes required for ureteric branching and up regulation of Tgfß2 and Dkk1.
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| Overall design |
Hoxb7-Cre:EGFP mice ( Zhao, et al. (2004) Dev Biol 276:403-415) were crossed with mice containing loxP sites flanking exon 3 of the ß-catenin allele (ß-catdelta3/delta3) (Harada,et al. (2002) Cancer Res 62:1971-1977) to generate ß-catenin gain-of-function mutant mice specific to the uteric bud, termed ß-catGOF-UB .Eighteen ß-catGOF-UB mutant kidneys and 9 WT kidneys were micro-dissected at E12.5. Mutant kidneys were divided into three random pools (n=3) consisting of 6 kidneys each and mRNA expression assessed by microarray.
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| Contributor(s) |
Bridgewater D, Cox B, Rosenblum N |
| Citation(s) |
21436291 |
| Submission date |
Feb 14, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Brian Joseph Cox |
| E-mail(s) |
b.cox@utoronto.ca
|
| Organization name |
University of Toronto
|
| Department |
Physiology
|
| Lab |
Cox System Biology
|
| Street address |
1 King's College Circle, Rm 3360
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM243346 |
normal mouse kidney E12.5, biological rep 1 |
| GSM243347 |
normal mouse kidney E12.5, biological rep 2 |
| GSM243348 |
normal mouse kidney E12.5, biological rep 3 |
| GSM509260 |
bcatGOF mouse kidney E12.5, biological rep 1 |
| GSM509261 |
bcatGOF mouse kidney E12.5, biological rep 2 |
| GSM509262 |
bcatGOF mouse kidney E12.5, biological rep 3 |
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| Relations |
| BioProject |
PRJNA125389 |
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