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Status |
Public on Apr 14, 2023 |
Title |
Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [ATAC-seq] |
Organism |
Rattus norvegicus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The Egr2 promoter antisense RNA (Egr2-AS-RNA) recruits chromatin remodeling complexes to inhibit Egr2 transcription following peripheral nerve injury. Here we show that the Egr2-AS-RNA modulates chromatin accessibility and serves as scaffold for two distinct histone modification complexes. The Egr2-AS-RNA binds to Ezh2 and Wdr5 and enables coordinate targeting of H3K27me3 and H3K4me3 to promoters of Egr2 and c-Jun. Expression of the AS-RNA results in reorganization of the global chromatin landscape and quantitative changes in loop formation and in contact frequency at domain boundaries exhibiting enrichment for AP-1 binding events. Our results show for the first time that the Egr2 promoter AS-RNA may serve as a regulator of coordinate chromatin remodeling and genome organization to regulate transcription, with a significant role in phenotypic plasticity of SCs.
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Overall design |
We performed ATAC-Seq on Schwann cells after ectopic expression of the Egr2-AS-RNA to determine changes in chromatin accessibility. Annotation of the peaks showed differential accessibility at some promoter regions, defined as the sequences from -1500 to +500 with respect to the transcription start site. TF footprinting showed an enrichment on AP-1 TF family.
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Contributor(s) |
Tapinos N, Fajardo JE, Karambizi D, Chen JS, Hwang H, Martinez-Moreno M |
Citation missing |
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Submission date |
Apr 26, 2022 |
Last update date |
Apr 16, 2023 |
Contact name |
Jorge Eduardo Fajardo |
E-mail(s) |
eduardo@fiserlab.org
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Organization name |
Albert Einstein College of Medicine
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Department |
Systems and Computational Biology
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Street address |
1300 Morris Park Avenue
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City |
Bronx |
State/province |
NY |
ZIP/Postal code |
10461 |
Country |
USA |
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Platforms (1) |
GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE201627 |
Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells |
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Relations |
BioProject |
PRJNA832335 |