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| Status |
Public on Dec 28, 2022 |
| Title |
Gene expression profiles during the process of differentiation of HL-60 AK2 hetero-knockout clone #5 cells into neutrophils [HL60AK2KO_clone5] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the changes in gene expression during the process of differentiation into neutrophils and to evaluate the effect of Ak2 hetero-knockout in the process, microarray analysis was performed.
HL-60 Ak2 hetero-knockout clone #5 cells were cultured with all-trans retinoic acid (ATRA) for 4 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.
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| Overall design |
HL-60 Ak2 hetero-knockout clone #5 cells were cultured with 10 µM ATRA and were collected at Day 0, 1, 2, and 4. Using prepared total RNA at each time point, microarray analysis was performed.
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| Contributor(s) |
Horiguchi T, Tanimura A, Miyoshi K, Hagita H, Noma T |
| Citation(s) |
36555730 |
| Submission date |
Apr 14, 2022 |
| Last update date |
Dec 29, 2022 |
| Contact name |
Taigo Horiguchi |
| E-mail(s) |
thoriguc1@gmail.com
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| Organization name |
Tokushima University
|
| Street address |
3-18-15, Kuramoto-cho
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| City |
Tokushima |
| ZIP/Postal code |
7708504 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE200827 |
Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils or eosinophils |
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| Relations |
| BioProject |
PRJNA826877 |
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