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| Status |
Public on Mar 30, 2022 |
| Title |
Comprehensive analysis for gene expressions of normal human dermal fibroblasts supplemented with N-acetylglucosamine -bearing polymers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To reveal the comprehensive gene expression of normal human dermal fibroblasts (NHDFs) based on the interaction of GlcNAc-bearing polymer, AC-GlcNAc10, with cell surface vimentin at 24 h, we performed a DNA microarray for NHDFs treated with AC-GlcNAc10. The DNA microarray analysis revealed altered expression of approximately 1000 genes in NHDF vs. NHDF treated with AC-GlcNAc10 by extracting differentially expressed genes with the ratio of >1.5 and <0.66-fold changes between the gene expressions of NHDF vs. NHDF treated with AC-GlcNAc10, and Z-score >2.0 and <-2.0. We observed the downregulation of gene expression related to cell cycle progression, such as baculoviral IAP repeat-containing protein 5 (BIRC5; survivin), and the upregulation of gene expression related to cell cycle arrest, such as cyclin-dependent kinase inhibitor 1A (CDKN1A; p21).
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| Overall design |
NHDFs are control. The data showed comparison between NHDFs (control) and AC-GlcNAc10-treated NHDFs
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| Contributor(s) |
Ise H |
| Citation(s) |
36190502 |
| Submission date |
Mar 29, 2022 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Hirohiko Ise |
| E-mail(s) |
ise@ms.ifoc.kyushu-u.ac.jp
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| Organization name |
Kyushu University
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| Department |
IMCE
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| Street address |
Motooka 744
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| City |
Fukuoka |
| ZIP/Postal code |
819-0395 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA821087 |
