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| Status |
Public on Apr 01, 2022 |
| Title |
Expression data from Orange cells (BT-OC) -treated HFDPCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals a potential role of Orange cells (BT-OC) in stimulating hair growth in dermal papilla cells.
HFDPCs were human primary cells line, treated with 1:2000 Orange cells (BT-OC) for 48 h. Microarray gene expression profiling was conducted for three biological replicates
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). Two hundred and fifty ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
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| Citation(s) |
35625924 |
| Submission date |
Mar 29, 2022 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Meriem Bejaoui |
| E-mail(s) |
mariembejaoui.mb@gmail.com
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| Organization name |
University of Tsukuba
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| Street address |
1 Chome-1-1 Tennodai
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| City |
Tsukuba, |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA821070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199651_BT-OC_vs_Control.txt.gz |
2.4 Kb |
(ftp)(http) |
TXT |
| GSE199651_RAW.tar |
13.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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