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| Status |
Public on Mar 26, 2022 |
| Title |
scRNA-seq of roof and floor endothelial cells at 21 hpf and 28 hpf in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to obtain the transcriptomic information about roof/dorsal and floor/ventral endothelial cells (ECs) of the dorsal aorta (DA) in zebrafish, we isolated single ECs from the DA roof and the floor respectively using the Tg(flk1:eGFP;flk1:NLS-Eos) zebrafish after photoconversion, and then performed scRNA sequencing. These single ECs were isolated at two different time points, 21 hpf and 28 hpf, when the lumen of the DA is established and the EHT begins , respectively.
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| |
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| Overall design |
Spatial photoconversion (DA roof versus DA floor), cell dissociation and mannual picking of photoconverted ECs
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| |
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| Contributor(s) |
Zhao S, Feng S, Tian Y, Wen Z |
| Citation(s) |
35333649 |
| Submission date |
Mar 02, 2022 |
| Last update date |
Mar 27, 2022 |
| Contact name |
Zhao Shizheng |
| E-mail(s) |
szhaoaf@connect.ust.hk
|
| Phone |
67608198
|
| Organization name |
HKUST
|
| Department |
LIFS
|
| Lab |
Wen's lab
|
| Street address |
HKUST
|
| City |
HongKong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (58)
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| Relations |
| BioProject |
PRJNA812001 |
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