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| Status |
Public on Jan 31, 2022 |
| Title |
RNA-Seq of 24 hours post fertilization zebrafish Kcng4b loss-of-function embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Comparison of transcriptome of 24 hours post fertilisation wild-type zebrafish embryos and that of embryos depleted of Kcng4b using anti-Kcng4b CRISPR (5’- GGCAGAGAAGGAATTCAGCA-3’, gRNA target site in exon 3) and splice site-blocking MO (5′-TGCATTCGCCCTGTAAAAGAACAAA-3′) targeting kcng4b intron 2-exon 3 (I2E3) junction.
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| Overall design |
Wild-type zebrafish embryos and that of embryos depleted of Kcng4b
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| Contributor(s) |
Shen H, Korzh V, Choi H |
| Citation missing |
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| Submission date |
Jan 24, 2022 |
| Last update date |
Jan 31, 2022 |
| Contact name |
Hyungwon Choi |
| E-mail(s) |
hyung_won_choi@nus.edu.sg
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| Organization name |
National University of Singapore
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| Lab |
Computational & Statistical Systems Biology
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| Street address |
14 Medical Drive, MD6 #08-01
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| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA800039 |
