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| Status |
Public on Apr 03, 2022 |
| Title |
Human prostate cancer cells (VCaP and AIVCaP): siAR alone (single siRNA) vs. siAR plus OPRK1 (combo-siRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Through comparative genomics using PDX models of androgen-dependent (AD) and castration-resistant (CR) tumors, we identify opioid receptor kappa 1 (OPRK1) as being associated with castration-resistance. Loss of OPRK1 function delays castration-resistance and inhibits castration-resistant growth of prostate cancer cells in culture and in vivo, suggesting OPRK1 as a therapeutic target.
To gain insight with regard to biological function of OPRK1 under androgen-depleted condition, we set up two distinct comparisons using VCaP and AIVCaP cells. AIVCaP is an androgen-independent subline of VCaP which we established. VCaP or AIVCaP were treated with siRNA for AR plus siRNA for OPRK1 (combo-siRNA) or siRNA for AR alone (single siRNA) (n = 4 each), and then expression profile gained from microarray analysis of the respective cell line treated with combo-siRNA was compared with that of cells treated with single siRNA.
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| Overall design |
Two cell lines, two-condition experiment: VCaP/AIVCaP treated with siAR alone (single siRNA) vs. siAR plus siOPRK1 (combo-siRNA). Biological replicates: 4 control (single siRNA) replicates, 4 combo-siRNA replicates.
*** For VCaP samples, the original raw files have been lost. ***
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| |
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| Contributor(s) |
Makino Y, Kobayashi T |
| Citation(s) |
35365763 |
| Submission date |
Jan 24, 2022 |
| Last update date |
Apr 05, 2022 |
| Contact name |
Yuki Makino |
| E-mail(s) |
yumakino@kuhp.kyoto-u.ac.jp
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| Organization name |
Kyoto University Graduate School of Medicine
|
| Department |
Urology
|
| Street address |
54 Shogoinkawahara-cho, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA799994 |
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