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| Status |
Public on Jan 15, 2022 |
| Title |
Investigation of differential microRNAs in the exosomes of alcohol-induced H9c2 myocardial cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We use the illumina high-throughput sequencing technology to identify miRNAs between the exosomes of H9c2 cells with or without alcohol-induced.The H9c2 cells were cultured in serum-free medium and stimulated with ethanol (100 mmol/L) or PBS for 24 h, then collected the exosomes samples from serum-free medium. Exosomes were isolated and extracted by differential centrifugation and detected by electron microscopy, particle size and related marker proteins.In total, 123 differentially expressed miRNAs (12 upregulated and 111 downregulated) were screened by miRNA sequence.
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| Overall design |
Examination of 2 different exosomes in H9c2 myocardial cells.
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| Contributor(s) |
Chen L, Meng Y, Hu Z |
| Citation missing |
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| Submission date |
Jan 12, 2022 |
| Last update date |
Jan 17, 2022 |
| Contact name |
Zhenzhen Hu |
| E-mail(s) |
201935808@mail.sdu.edu.cn
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| Organization name |
Shandong University
|
| Street address |
Lixia District
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| City |
Jinan |
| ZIP/Postal code |
250014 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (2) |
| GSM5814011 |
exosomes of alcohol-induced H9c2 myocardial cells |
| GSM5814012 |
exosomes of normal H9c2 myocardial cells |
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| Relations |
| BioProject |
PRJNA796572 |
