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| Status |
Public on Jan 15, 2022 |
| Title |
Comprehensive analysis of circRNA expression profiles in rat cerebral cortex after moderate traumatic brain injury |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Traumatic brain injury is a medical event of global concern, and a growing body of research suggests that circular RNA can play very important roles in traumatic brain injury. To explore the functions of more novel and valuable circular RNA in traumatic brain injury response, a moderate traumatic brain injury in rat was established and a comprehensive analysis of circular RNA expression profiles in rat cerebral cortex was done.
Results: As a result, 301 up-regulated and 284 down-regulated circular RNAs were obtained in moderate traumatic brain injury rats, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed based on the circular RNA’s host genes, and a circRNA-miRNA interaction network based on differentially expressed circular RNAs was constructed. Also, four circular RNAs were validated by RT-qPCR and sanger sequencing.
Conclusion: This study showed that differentially expressed circular RNAs existed between rat cerebral cortex after moderate traumatic brain injury and control. And this will provide valuable information for circular RNA research in the field of traumatic brain injury.
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| Overall design |
Cerebral cortex circular RNA profiles of sham and mTBI (moderate traumatic brain injury) rat
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| Contributor(s) |
Li G, Li S, Liu R, Yu J, Ma H, Zhao Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 12, 2022 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Gang Li |
| E-mail(s) |
whu_ligang@whu.edu.cn
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| Organization name |
Zhongnan Hospital of Wuhan University
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| Street address |
169 Donghu Road, Wuchang District
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| City |
wuhan |
| ZIP/Postal code |
430071 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA796539 |
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