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| Status |
Public on Dec 30, 2021 |
| Title |
Hepatocytes :MH vs. ProliHH vs. PHH vs. HLC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hepatocytes isolated from DILI patient's liver (#2064) were cultured for a long term using irrMEF and EMUKK-05, and comprehensive gene expression was compared between Puromycin-treated and non-treated groups. In addition, comprehensive gene expression analysis of human mature hepatocytes, primary cultured cells, and ips cell-derived hepatocyte-like cells were performed as controls.
Two-condition experiment, Proliferating hepatocytes (ProilHH) vs. puromycin-treated ProliHH. Primary human hepatocytes (PHH) and isolated humen mature hepatocytes (MH) and human iPSC-derived hepatocyte-like cells (HLC) as controls.
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| Overall design |
Hepatocytes were isolated from the livers of human DILI patients and frozen after primary culture. The cells were cultured for a long turm of time in MEF feeder and EMUKK-05 medium (ProliHH), and the gene expression changes between the puromycin- and non-puromoted groups were analyzed. Puromycin was used as a selective agent for hepatocytes.
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| Contributor(s) |
Akiyama S, Saku N, Miyata S, Ite K, Toyoda M, Kimura T, Kuroda M, Nakazawa A, Kasahara M, Nonaka H, Kamiya A, Kiyono T, Kobayashi T, Murakami Y, Umezawa A |
| Citation(s) |
35279203 |
| Submission date |
Dec 27, 2021 |
| Last update date |
Apr 05, 2022 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
umezawa@1985.jukuin.keio.ac.jp
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| Phone |
81-3-5494-7047
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| Organization name |
National Center for Child Health and Development
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| Department |
Center for Regenerative Medicine
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| Street address |
2-10-1 Okura
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| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA792566 |
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