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| Status |
Public on Aug 22, 2010 |
| Title |
Global gene expression profiles among five independent cytoplasmic male sterile lines. |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
|
| Summary |
We employed a microarray-based transcriptomic status comparison of rice cytoplasmic male sterile (CMS) lines in order to categorize the nuclear gene expressions upon cytoplasmic substitution. In anthers at the uninucleate and bicellular pollen stages, we found that 8,199 genes significantly changed their expression in at least one of the CMS lines. We categorized the genes into 100 clusters by k-means clustering, and common obvious expression patterns were observed in W11, LD and BT.
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| Overall design |
RNA expression was measured for the anthers at uninucleate and bicellular pollen stages of five CMS lines (BT, CW, LD, W11, WA). T65 was used as the wild-type control. Microarray analysis was performed by three replicates using RNA extracted from independent plants.
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| Contributor(s) |
Fujii S, Fujita M, Kurata N, Toriyama K |
| Citation(s) |
20203238 |
| Submission date |
Sep 10, 2009 |
| Last update date |
Jan 09, 2014 |
| Contact name |
Kinya Toriyama |
| E-mail(s) |
torikin@bios.tohoku.ac.jp
|
| Phone |
81-22-717-8830
|
| Fax |
81-22-717-8834
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| URL |
http://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?English
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| Organization name |
Tohoku University
|
| Department |
Graduate School of Agricultural Science
|
| Lab |
Laboratory of Environmental Plant Biotechnology
|
| Street address |
Tsutsumidori^Amamiyamachi, Aobaku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
981-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL8852 |
Agilent-015241 Rice Gene Expression 4x44K Microarray (Probe name version) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA119319 |
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