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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 20, 2004 |
| Title |
Control and starved myotubes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal Bovine serum, Invitrogen Corporation, Carlsbad, CA) in 5% CO2. Cells were plated at a density of 40-50% in 100mm dishes. When cells reached ~90% confluency 24 hours later, they were switched to differentiation media (DMEM containing 2% horse serum; GIBCO, Invitrogen Corporation, Carlsbad, CA) which was subsequently changed every 48 hours. All cells were allowed to differentiate for 4 days with feeding every two days (day 0, 2, and 4). Beginning at day 4, control cells were re-fed every 48 hours whereas experimental cells were not re-fed. After an additional 4 days (8 days post differentiation), cells were harvested and total RNA was isolated from control and "starved" (non re-fed cells)
Keywords: repeat sample
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| Contributor(s) |
Stevenson EJ, Koncarevic A, Giresi PG, Jackman RW, Kandarian SC |
| Citation(s) |
15608089 |
| Submission date |
Sep 17, 2004 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Susan Kandarian |
| E-mail(s) |
skandar@bu.edu
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| Phone |
617-353-7493
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| Organization name |
Boston University
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| Department |
Department of Health Sciences
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| Street address |
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA90567 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE1776_RAW.tar |
29.2 Mb |
(http)(custom) |
TAR (of CEL) |
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