|
| Status |
Public on Nov 10, 2021 |
| Title |
Integrated molecular analysis identifies new developmental pericyte markers in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single cell and bulk RNA-seq analyses on pdgfrb-positive cells to identify candidate pericyte-expressed genes. For bulk RNA-seq, we used wild type pdgfrb-positive samples from Series GSE152759 (GEO ID: GSM4625924, GSM4625925, GSM4625926) for comparison to mutant pdgfrb-positive cells in this Series. These cells had originally been isolated in parallel.
|
| |
|
| Overall design |
RNA-seq was performed on transgene-positive cells isolated by FACS.
|
| |
|
| Contributor(s) |
Lawson N |
| Citation(s) |
34751773 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 HL140017 |
Embryonic origins of endothelial heterogeneity |
UNIV OF MASSACHUSETTS MED SCHOOL |
NATHAN D LAWSON |
|
| Submission date |
Jun 03, 2021 |
| Last update date |
Nov 12, 2021 |
| Contact name |
Nathan D. Lawson |
| E-mail(s) |
nathan.lawson@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell, and Cancer Biology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA734950 |
| SRA |
SRP322535 |
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