|
| Status |
Public on May 08, 2024 |
| Title |
Single-cell RNA sequencing of adult mouse fibrotic kidney induced by unilateral uretral obstruction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single-cell RNAseq for adult mouse fibrotic kidney to characheterize how epithelial to mesenchymal transition program is implemented at single tubular cells resolution during the course of renal intrestitial fibrosis
|
| |
|
| Overall design |
12 weeks old males mice were subjected to SHAM sergery (CTR) or to unilateral ureteral obstruction (UUO) to indice renal interstitial fibrosis. 1UUO and SHAM kidneys were harvested and disregated into single cell preparations contaning all kidney cell types. 10X Chromium were used to prepare cDNA libraries. Two UUO and one SHAM cDNA libraries were obtained sequenced.
|
| |
|
| Contributor(s) |
Narwade N, Youssef KK, Nieto A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 24, 2021 |
| Last update date |
May 08, 2024 |
| Contact name |
Angela Nieto |
| E-mail(s) |
anieto@umh.es
|
| Organization name |
Instituto de Neurociencias (CSIC-UMH)
|
| Department |
Developmental Neurobiology
|
| Lab |
Cell plasticity in development and disease
|
| Street address |
Av. Ramón y Cajal s/n
|
| City |
San Juan de Alicante |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA732362 |
| SRA |
SRP321104 |
